Non-Invasive Functional Neuroimaging in Mice Using Diffuse Optical Tomography

Matt Reisman, Department of Physics, Washington University
September 22, 2017 at 4:00 pm
204 Crow
Event Description 

As advances in functional MRI have transformed the study of human brain function, they have also widened the divide between standard research techniques used in humans and those used in mice, where high quality images are difficult to obtain using fMRI given the small volume of the mouse brain. Optical imaging techniques have been developed to study mouse brain networks, which are highly valuable given the ability to study brain disease treatments or development in a controlled environment, but traditional methods are somewhat invasive and are limited to imaging a two-dimensional slice of the cortex. Diffuse Optical Tomography (DOT) is a non-invasive volumetric imaging technique with many applications towards human clinical imaging, but its applications to rodent imaging are either too slow or too sparsely sampling to observe full brain hemodynamics at good resolution. We have developed a technique incorporating patterned illumination of the whole head instead of the more typ ical point illumination, called Structured Illumination (SI), into traditional DOT imaging to increase its speed, spatial sampling, and overall flexibility. This provides non-invasive, 3D imaging of brain function in mice. This talk will discuss optical methods as a general imaging modality, the implementation of structured illumination into traditional DOT techniques, and the construction, optimization, and applications of a large field-of-view structured illumination DOT imaging system.